Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production
Identifieur interne : 002272 ( Main/Exploration ); précédent : 002271; suivant : 002273Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production
Auteurs : András Hrabák [Hongrie] ; Vincent Vercruysse [Belgique] ; Ilona L. Kahán [Hongrie] ; Bernard Vray [Belgique]Source :
- Life Sciences [ 0024-3205 ] ; 2001.
English descriptors
- Teeft :
- Arginase, Arginase activity, Cyclooxygenase, European journal, Indomethacin, Inducible, Inducible nitric oxide synthase, Inhibitor, Inhibitory effect, Life sciences, Macrophage, Mouse macrophages, Murine, Murine macrophages, Nitric, Nitric oxide, Nitric oxide production, Nitrite, Nitrite production, Oxide, Peritoneal macrophages, Semmelweis university, Synthase, Uridine, Uridine incorporation, Various concentrations.
Abstract
Abstract: Indomethacin (0.1–0.5 mM concentration) inhibits nitric oxide production in murine peritoneal macrophages. This was evidenced by measuring both nitrite production or 14C-L-citrulline formation. The inhibition was caused by the diminution of de novo inducible nitric oxide synthase production as demonstrated by Western blotting experiment. The effect of indomethacin after 4 h treatment was irreversible. NO synthase and arginase activities and the uptake of arginine were not directly affected by the drug. Indomethacin also decreased uridine incorporation in macrophages. The effect of indomethacin on the induction of other enzymes (i.e. arginase) was weaker.
Url:
DOI: 10.1016/S0024-3205(01)00978-X
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001D02
- to stream Istex, to step Curation: 001D02
- to stream Istex, to step Checkpoint: 001095
- to stream Main, to step Merge: 002296
- to stream Main, to step Curation: 002272
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production</title><author><name sortKey="Hrabak, Andras" sort="Hrabak, Andras" uniqKey="Hrabak A" first="András" last="Hrabák">András Hrabák</name></author><author><name sortKey="Vercruysse, Vincent" sort="Vercruysse, Vincent" uniqKey="Vercruysse V" first="Vincent" last="Vercruysse">Vincent Vercruysse</name></author><author><name sortKey="Kahan, Ilona L" sort="Kahan, Ilona L" uniqKey="Kahan I" first="Ilona L." last="Kahán">Ilona L. Kahán</name></author><author><name sortKey="Vray, Bernard" sort="Vray, Bernard" uniqKey="Vray B" first="Bernard" last="Vray">Bernard Vray</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:07D0F4AD78ABD839914D160A40E534D3B989C299</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0024-3205(01)00978-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-3Q0NXKC8-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001D02</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D02</idno><idno type="wicri:Area/Istex/Curation">001D02</idno><idno type="wicri:Area/Istex/Checkpoint">001095</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001095</idno><idno type="wicri:doubleKey">0024-3205:2001:Hrabak A:indomethacin:prevents:the</idno><idno type="wicri:Area/Main/Merge">002296</idno><idno type="wicri:Area/Main/Curation">002272</idno><idno type="wicri:Area/Main/Exploration">002272</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production</title><author><name sortKey="Hrabak, Andras" sort="Hrabak, Andras" uniqKey="Hrabak A" first="András" last="Hrabák">András Hrabák</name><affiliation wicri:level="3"><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Hongrie</country></affiliation></author><author><name sortKey="Vercruysse, Vincent" sort="Vercruysse, Vincent" uniqKey="Vercruysse V" first="Vincent" last="Vercruysse">Vincent Vercruysse</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Experimental Immunology, Faculty of Medicine, Free University of Brussels (ULB), Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author><author><name sortKey="Kahan, Ilona L" sort="Kahan, Ilona L" uniqKey="Kahan I" first="Ilona L." last="Kahán">Ilona L. Kahán</name><affiliation wicri:level="3"><country xml:lang="fr">Hongrie</country><wicri:regionArea>2nd Department of Ophthalmology, Semmelweis University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Vray, Bernard" sort="Vray, Bernard" uniqKey="Vray B" first="Bernard" last="Vray">Bernard Vray</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Experimental Immunology, Faculty of Medicine, Free University of Brussels (ULB), Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Life Sciences</title><title level="j" type="abbrev">LFS</title><idno type="ISSN">0024-3205</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">68</biblScope><biblScope unit="issue">16</biblScope><biblScope unit="page" from="1923">1923</biblScope><biblScope unit="page" to="1930">1930</biblScope></imprint><idno type="ISSN">0024-3205</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0024-3205</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Arginase</term><term>Arginase activity</term><term>Cyclooxygenase</term><term>European journal</term><term>Indomethacin</term><term>Inducible</term><term>Inducible nitric oxide synthase</term><term>Inhibitor</term><term>Inhibitory effect</term><term>Life sciences</term><term>Macrophage</term><term>Mouse macrophages</term><term>Murine</term><term>Murine macrophages</term><term>Nitric</term><term>Nitric oxide</term><term>Nitric oxide production</term><term>Nitrite</term><term>Nitrite production</term><term>Oxide</term><term>Peritoneal macrophages</term><term>Semmelweis university</term><term>Synthase</term><term>Uridine</term><term>Uridine incorporation</term><term>Various concentrations</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Indomethacin (0.1–0.5 mM concentration) inhibits nitric oxide production in murine peritoneal macrophages. This was evidenced by measuring both nitrite production or 14C-L-citrulline formation. The inhibition was caused by the diminution of de novo inducible nitric oxide synthase production as demonstrated by Western blotting experiment. The effect of indomethacin after 4 h treatment was irreversible. NO synthase and arginase activities and the uptake of arginine were not directly affected by the drug. Indomethacin also decreased uridine incorporation in macrophages. The effect of indomethacin on the induction of other enzymes (i.e. arginase) was weaker.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>Hongrie</li></country><region><li>Hongrie centrale</li><li>Région de Bruxelles-Capitale</li></region><settlement><li>Bruxelles</li><li>Budapest</li></settlement></list><tree><country name="Hongrie"><region name="Hongrie centrale"><name sortKey="Hrabak, Andras" sort="Hrabak, Andras" uniqKey="Hrabak A" first="András" last="Hrabák">András Hrabák</name></region><name sortKey="Hrabak, Andras" sort="Hrabak, Andras" uniqKey="Hrabak A" first="András" last="Hrabák">András Hrabák</name><name sortKey="Kahan, Ilona L" sort="Kahan, Ilona L" uniqKey="Kahan I" first="Ilona L." last="Kahán">Ilona L. Kahán</name></country><country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Vercruysse, Vincent" sort="Vercruysse, Vincent" uniqKey="Vercruysse V" first="Vincent" last="Vercruysse">Vincent Vercruysse</name></region><name sortKey="Vray, Bernard" sort="Vray, Bernard" uniqKey="Vray B" first="Bernard" last="Vray">Bernard Vray</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002272 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002272 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:07D0F4AD78ABD839914D160A40E534D3B989C299
|texte= Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production
}}
| This area was generated with Dilib version V0.6.33. |  |